PSEUDO-ISOENZYME FORMS OF LIVER PRENYL TRANSFERASE

被引:20
作者
BARNARD, GF [1 ]
LANGTON, B [1 ]
POPJAK, G [1 ]
机构
[1] UNIV CALIF LOS ANGELES,INST MOLEC BIOL,LOS ANGELES,CA 90024
关键词
D O I
10.1016/0006-291X(78)90655-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Prenyl transferase of pig liver exists in two forms, A and B, the latter being more acidic than form A. The ratio of these two forms in initial extracts of the organ varies according to the presence or absence of a thiol reducing agent in the extraction medium. In the presence of 10 mM β-mercaptoethanol the two forms exist as an equilibrium mixture of ca. 70% form A and 30% form B. The latter, when separated and rechromatographed with β-mercaptoethanol reverts to approximate the same 7:3 ratio of forms A and B. In the absence of a thiol reducing agent form B does not change, but form A may be converted almost completely to form B after treatment with oxidized glutathione. © 1978.
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页码:1097 / 1103
页数:7
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