MONOCLONAL-ANTIBODIES AGAINST DEFINED REGIONS OF THE MUSCULAR-DYSTROPHY PROTEIN, DYSTROPHIN

Nineteen monoclonal antibodies which bind to native dystrophin in the plasma membrane of frozen muscle sections were obtained using a recombinant fusion protein as immunogen. On Western blots of normal mouse muscle extracts, the antibodies bind specifically to a 400 000 Mr protein which is absent from dystrophic mouse (mdx) muscle. At least four distinct epitopes have been identified by cleavage mapping methods. Although the fusion protein contained 25% of the human dystrophin sequence (Cys816-Asp1747; Mr 108 000), most of the monoclonal antibodies (15 out of 19) recognize a single fragment of Mr 27 500. © 1990.