IMPAIRED INDUCTION OF VASOACTIVE INTESTINAL POLYPEPTIDE AFTER SCIATIC-NERVE INJURY IN THE STREPTOZOTOCIN-DIABETIC RAT

被引：13
作者：
CALCUTT, NA [1 ]
MIZISIN, AP [1 ]
YAKSH, TL [1 ]
机构：
[1] UNIV CALIF SAN DIEGO,DEPT ANESTHESIOL,LA JOLLA,CA 92093
来源：
JOURNAL OF THE NEUROLOGICAL SCIENCES | 1993年 / 119卷 / 02期
关键词：
VASOACTIVE INTESTINAL POLYPEPTIDE; DIABETIC NEUROPATHY; AXONAL TRANSPORT; REGENERATION; CRUSH INJURY;
D O I：
10.1016/0022-510X(93)90128-L
中图分类号：
R74 [神经病学与精神病学];
学科分类号：
摘要：
This study was designed to assess the effects of experimental diabetes and nerve crush injury upon vasoactive intestinal polypeptide (VIP) content and axonal transport in the dorsal root ganglia (DRG) and sciatic nerve. Sciatic nerve crush injury in control and 3-week streptozotocin-diabetic rats was followed 6.5 days later by placement of 2 constricting ligatures above the site of injury. After 12 h, the L4 and L5 DRG and sciatic nerve were removed for VIP radioimmunoassay. Similar samples were also taken from control and diabetic rats whose nerve had been ligated without a preceding crush. VIP was increased over 2-fold in ganglia and 4-fold in nerves of crush-injured controls compared to uninjured controls (both P < 0.01). Crush injury also increased ganglion and nerve VIP in diabetic rats (P < 0.05 and 0.01, respectively) but the increase was less than what occurred in crush-injured controls (both P < 0.05). The accumulation of VIP proximal to a sciatic ligature was similar in control and diabetic rats and was not altered in either group by crush injury, while retrograde transport of VIP was initiated by crush injury in both control and diabetic rats. These data show that short-term diabetes does not alter the amount and peripheral axonal transport of VIP in the sciatic nerve but impairs the ability of peripheral nerve to respond to injury.
引用
收藏
页码：154 / 161
页数：8
相关论文
共 64 条
[1]   STUDIES OF VASOACTIVE INTESTINAL POLYPEPTIDE EXPRESSION IN INJURED PERIPHERAL NEURONS USING CAPSAICIN, SYMPATHECTOMY AND MF MUTANT RATS [J].
ANAND, P ;
GIBSON, SJ ;
SCARAVILLI, F ;
BLANK, MA ;
MCGREGOR, GP ;
APPENZELLER, O ;
DHITAL, K ;
POLAK, JM ;
BLOOM, SR .
NEUROSCIENCE LETTERS, 1990, 118 (01) :61-66
[2]   WATER-CONTENT, VASOACTIVE INTESTINAL POLYPEPTIDE AND SUBSTANCE-P IN INTACT AND CRUSHED SCIATIC-NERVES OF NORMAL AND STREPTOZOTOCIN-DIABETIC RATS [J].
ANAND, P ;
LLEWELYN, JG ;
THOMAS, PK ;
GILLON, KRW ;
LISK, R ;
BLOOM, SR .
JOURNAL OF THE NEUROLOGICAL SCIENCES, 1988, 83 (2-3) :167-177
[3]   ENTERIC NERVES IN DIABETIC RATS - INCREASE IN VASOACTIVE INTESTINAL POLYPEPTIDE BUT NOT SUBSTANCE-P [J].
BELAI, A ;
LINCOLN, J ;
MILNER, P ;
CROWE, R ;
LOESCH, A ;
BURNSTOCK, G .
GASTROENTEROLOGY, 1985, 89 (05) :967-976
[4]   RESTORATION OF DIABETES-INDUCED CHANGES IN ENTERIC NERVES BY PHORBOL 12,13-DIBUTYRATE [J].
BELAI, A ;
BURNSTOCK, G .
BRAIN RESEARCH, 1992, 575 (02) :356-358
[5]  
BISBY MA, 1980, EXP NEUROL, V69, P74, DOI 10.1016/0014-4886(80)90144-2
[6]   AXONAL-TRANSPORT OF SUBSTANCE P-LIKE IMMUNOREACTIVITY IN REGENERATING RAT SCIATIC-NERVE [J].
BISBY, MA ;
KEEN, P .
BRAIN RESEARCH, 1985, 361 (1-2) :396-399
[7]   REGENERATION OF PRIMARY AFFERENT NEURONS CONTAINING SUBSTANCE P-LIKE IMMUNOREACTIVITY [J].
BISBY, MA ;
KEEN, P .
BRAIN RESEARCH, 1986, 365 (01) :85-95
[8]   AXONAL-TRANSPORT OF SUBSTANCE-P-LIKE IMMUNOREACTIVITY IN GANGLIOSIDE-TREATED DIABETIC RATS [J].
CALCUTT, NA ;
TOMLINSON, DR ;
WILLARS, GB ;
KEEN, P .
JOURNAL OF THE NEUROLOGICAL SCIENCES, 1990, 96 (2-3) :283-291
[9]   AN INCREASE OF VASOACTIVE INTESTINAL POLYPEPTIDE-CONTAINING, BUT NOT NEUROPEPTIDE-Y-CONTAINING, SUBSTANCE-P-CONTAINING OR CATECHOLAMINE-CONTAINING NERVES IN THE IRIS OF THE STREPTOZOTOCIN-INDUCED DIABETIC RAT [J].
CROWE, R ;
BURNSTOCK, G .
EXPERIMENTAL EYE RESEARCH, 1988, 47 (05) :751-759
[10]   DEPLETION OF SUBSTANCE-P AND CALCITONIN GENE-RELATED PEPTIDE IN SCIATIC-NERVE OF RATS WITH EXPERIMENTAL DIABETES - EFFECTS OF INSULIN AND ALDOSE REDUCTASE INHIBITION [J].
DIEMEL, LT ;
STEVENS, EJ ;
WILLARS, GB ;
TOMLINSON, DR .
NEUROSCIENCE LETTERS, 1992, 137 (02) :253-256
1234567