SEQUENCE-ANALYSIS OF THE RDNA SPACER OF PARACENTROTUS-LIVIDUS AND OBSERVATIONS ABOUT PRE-RIBOSOMAL-RNA PROCESSING - NTS SEQUENCE OF PARACENTROTUS-LIVIDUS RDNA

被引:16
|
作者
CANTONE, M
BARBIERI, R
DURO, G
IZZO, V
GIUDICE, G
机构
[1] UNIV PALERMO,DIPARTIMENTO BIOL CELLULARE & SVILUPPO,VIA ARCHIRAFI 22,I-90134 PALERMO,ITALY
[2] CNR,IST BIOL SVILUPPO,I-90123 PALERMO,ITALY
关键词
EXTERNAL TRANSCRIBED SPACER; NONTRANSCRIBED SPACER; NORTHERN-BLOT HYBRIDIZATION; RIBOSOMAL RNA; SEA URCHIN; S1; MAPPING;
D O I
10.1007/BF01674428
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have isolated and sequenced one intergenic region and a small part of the flanking regions (18S and 26S rRNA coding regions) of the rRNA-encoding genes (rDNA) from the sea urchin Paracentrotus lividus. This region is about 3.8 Kb long. Northern blot hybridizations and S1 mapping experiments demonstrated the presence of a partially processed 21S rRNA precursor which has the same 5' terminus as the 32S primary precursor, also in developmental stages characterized by a low rate of rRNA synthesis.
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页码:177 / 182
页数:6
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