ISOLATION AND IDENTIFICATION OF A NEW DIURETIC PEPTIDE FROM THE TOBACCO HORNWORM, MANDUCA-SEXTA

被引：82

作者：

BLACKBURN, MB

KINGAN, TG

BODNAR, W

SHABANOWITZ, J

HUNT, DF

KEMPE, T

WAGNER, RM

RAINA, AK

SCHNEE, ME

MA, MC

机构：

[1] UNIV MARYLAND,DEPT ENTOMOL,COLLEGE PK,MD 20742

[2] USDA ARS,BELTSVILLE AGR RES CTR,INSECT NEUROBIOL & HORMONE LAB,BELTSVILLE,MD 20705

[3] UNIV VIRGINIA,DEPT CHEM,CHARLOTTESVILLE,VA 22901

[4] UNIV MARYLAND,DEPT CHEM & BIOCHEM,COLLEGE PK,MD 20742

[5] USDA ARS,BELTSVILLE AGR RES CTR,LIVESTOCK INSECT LAB,BELTSVILLE,MD 20705

[6] DUPONT CO,AGR PROD,STINE HASKELL RES CTR,NEWARK,DE 19714

[7] UNIV MARYLAND,MARYLAND BIOTECHNOL INST,CTR AGR BIOTECHNOL,COLLEGE PK,MD 20742

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 181卷 / 03期

关键词：

D O I：

10.1016/0006-291X(91)92025-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 30-amino acid diuretic peptide was isolated from the corpora cardiaca-corpora allata complexes and, separately, from medial neurosecretory cells of the Sphingid moth, Manduca sexta. The peptide was found to have the following sequence, determined by automated Edman degradation and mass spectrometry: SFSVNPAVDILQHRYMEKV AQNNRNFLNRV-NH2. We have named the peptide Mas-DP II. The peptide was synthesized and shown to possess diuretic activity in decapitated moths. Mas-DP II is related by sequence homology to a 41-amino acid diuretic peptide identified previously from M. sexta, and it belongs to the family of corticotropin releasing factor-like peptides. © 1991.

引用

页码：927 / 932

页数：6

共 20 条

[1] THE ROLE OF THE FRONTAL GANGLION AND CORPORA CARDIACA CORPORA ALLATA COMPLEX IN POST-FEEDING WEIGHT-LOSS IN ADULT HELIOTHIS-ZEA [J].