CALIBRATION MIXTURE FOR ESTIMATION OF PEPTIDE MOLECULAR-WEIGHT BY EXCLUSION CHROMATOGRAPHY

被引：10

作者：

WILGUS, H

STELLWAGEN, E

机构：

[1] Department of Biochemistry, University of Iowa, Iowa City

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 94卷 / 01期

关键词：

D O I：

10.1016/0003-2697(79)90813-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A procedure is described for the rapid preparation of a partial cyanogen bromide digest of horse heart cytochrome c. This digest serves as a calibration mixture for estimation of peptide molecular weight in the range 500 to 20,000 by exelusion chromatography in denaturing solvents. The elution profile of the calibration mixture from Sephadex G-50 equilibrated and developed with 10% formic acid is described by the relationship log M = 4.79 - 0.42 ( Vo Vo). © 1979.

引用

页码：228 / 230

页数：3