THE 3 HEAVY-CHAIN PRECURSORS FOR THE INTER-ALPHA-INHIBITOR FAMILY IN MOUSE - NEW MEMBERS OF THE MULTICOPPER OXIDASE PROTEIN GROUP WITH DIFFERENTIAL TRANSCRIPTION IN LIVER AND BRAIN

被引:53
作者
CHAN, P
RISLER, JL
RAGUENEZ, G
SALIER, JP
机构
[1] INST FED RECH MULTIDISCIPLINAIRES PEPTIDES,BOIS GUILLAUME,FRANCE
[2] CNRS,CTR GENET MOLEC,GIF SUR YVETTE,FRANCE
关键词
D O I
10.1042/bj3060505
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The inter-alpha-inhibitor (I alpha I) family is comprised of the plasma protease inhibitors I alpha I, inter-alpha-like inhibitor (I alpha LI), pre-alpha-inhibitor (P alpha I) and bikunin. I alpha I, I alpha LI and P alpha I are distinct assemblies of bikunin with one of three heavy (H) chains designated H1, H2 and H3. These H chains and bikunin are respectively encoded by a set of three H genes and an alpha(1)-microglobulin/bikunin precursor (AMBP) gene. All four gene products undergo maturation steps from precursor polypeptides. The full-length cDNAs for the H1-, H2- and H3-chain precursors were cloned from a mouse liver cDNA library and sequenced. Extensive searches of amino acid sequence similarities to other proteins in databanks revealed (i) a highly significant similarity of the C-terminal sequence in the three H-chain precursors to the multicopper-binding domain in the group of multicopper oxidase proteins and (ii) the presence of von Willebrand type-A domains in the mature H chains. Amino acid sequence comparisons between the three mouse H1-, H2- and H3-chain precursors and their human counterparts allowed us to appraise the timing and order of occurrence of the three H-chain genes from a shared ancestor during mammalian evolution. Owing to a multiple alignment of the six mouse and human nucleotide sequences for these H-chain precursors, a reverse transcriptase PCR assay with degenerate oligonucleotides was designed, allowing us to (i) present evidence that no mRNAs for further H genes exist in mouse liver and (ii) demonstrate a previously undescribed transcription of the H2- and H3-chain mRNAs in mouse brain, which contrasts with the expression of all four, H1, H2, H3 and AMBP, mRNAs in liver.
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页码:505 / 512
页数:8
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