PROTOPLAST FUSION MEDIATED TRANSFER OF OLIGOMYCIN RESISTANCE FROM NICOTIANA-SYLVESTRIS TO SOLANUM-TUBEROSUM BY INTERGENERIC HYBRIDIZATION

被引：10

作者：

PERL, A ^{[1
]}

AVIV, D ^{[1
]}

GALUN, E ^{[1
]}

机构：

[1] WEIZMANN INST SCI,DEPT PLANT GENET,IL-76100 REHOVOT,ISRAEL

来源：

MOLECULAR & GENERAL GENETICS | 1991年 / 225卷 / 01期

关键词：

PROTOPLAST FUSION; OLIGOMYCIN RESISTANCE; INTERGENERIC HYBRIDIZATION; NICOTIANA; SOLANUM;

D O I：

10.1007/BF00282636

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have successfully bridged the intergeneric barriers between Nicotiana and Solanum with respect to chondriome transfer. To enable this transfer we utilized the donor-recipient protoplast-fusion procedure. Consequently protoplasts of a Nicotiana sylvestris line with putatively oligomycin-resistant mitochondria (line Oli(R)38) were used as irradiated chondriome donors and idodoacetate-treated protoplasts to Solanum tuberosum cv. Desiree served as recipients. The plated fusion products as well as their derived colonies and calli were exposed to gradually increasing levels of oligomycin. The resulting plantlets had potato morphology and were analyzed with respect to their mitochondrial DNA and chloroplast DNA. Fifteen out of 50 regenerated plants were verified as true cybrids. Detailed analyses of one cybrid revealed chondriome components from the oligomycin-resistant donor line, Oli(R)38, but retention of the plastome of potato. This cybrid was oligomycin-resistant as revealed by root-culture analysis. It was thus verified that due to selection, chondriome components could be transferred from a N. sylvestris donor into a cybrid having all the phenotypic features controlled by the nucleus of the recipient fusion partner (S. tuberosum).

引用

页码：11 / 16

页数：6

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