PURIFICATION AND CHARACTERIZATION OF THE HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE FROM SACCHAROMYCES-CEREVISIAE

被引：18

作者：

SCHMIDT, R ^{[1
]}

WIEGAND, H ^{[1
]}

REICHERT, U ^{[1
]}

机构：

[1] FREE UNIV BERLIN, FACHBEREICH BIOL, INST BIOCHEM & MOLEC BIOL, D-1000 BERLIN 33, GERMANY

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1979年 / 93卷 / 02期

关键词：

D O I：

10.1111/j.1432-1033.1979.tb12830.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. Hypoxanthine‐guanine phosphoribosyltransferase (EC 2.4.2.8) from Saccharomyces cerevisiae was purified 9400‐fold by affinity chromatography giving rise to an electrophoretically homogeneous preparation. 2. The molecular weight of the enzyme was determined by gel filtration with Sephadex G‐100 and by sodium dodecylsulfate gel electrophoresis. Both methods reveal a molecular weight of 51000. 3. The enzyme requires Mg2+ and has its pH optimum at 8.5. 4. Isoelectric focussing as well gel electrophoresis of the purified extract reveals a single band which exhibits enzyme activity. The isoelectric point of the enzyme is 5.1. 5. The enzyme displays Michaelis‐Menten kinetics with apparent Michaelis constants for hypoxanthine, guanine and phosphoribosylpyrophosphate of 23 μM, 18 μM, and 50 μM respectively. Copyright © 1979, Wiley Blackwell. All rights reserved

引用

页码：355 / 361

页数：7

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