IDENTIFICATION OF MUTANS STREPTOCOCCI WITH MONOCLONAL-ANTIBODIES

被引：19

作者：

DESOET, JJ ^{[1
]}

VANDALEN, PJ ^{[1
]}

RUSSELL, RRB ^{[1
]}

DEGRAAFF, J ^{[1
]}

机构：

[1] UNIV LONDON LONDON HOSP,COLL MED,HUNTERIAN DENT RES UNIT,LONDON E1 2AD,ENGLAND

来源：

ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY | 1990年 / 58卷 / 04期

关键词：

IDENTIFICATION; MONOCLONAL ANTIBODIES; MUTANS; STREPTOCOCCI; S-SOBRINUS; STREPTOCOCCUS-MUTANS;

D O I：

10.1007/BF00399332

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Mutans streptococci have been correlated with dental caries. The identification of the species within this group is still a problem. The characterization of a monoclonal antibody (Mab) OMVU10 against S. sobrinus as well as the isolation and characterization of Mabs against S. mutans (OMVU30 and OMVU31), S. cricetus (OMVU40) and mutans streptococci (OMVU2) is described. The epitope specificity for OMVU10 and OMVU31 was cell-wall antigen B in both cases although both Mabs recognized different species-specific epitopes. OMVU40 was cross reactive with Steptococcus sanguis taxon 3. All other Mabs were specific for one species. Using these Mabs, a key to the identification of mutans streptococci is developed. This key was tested for 85 wild type isolates of mutans streptococci and proved to be highly reliable and easy to perform.

引用

页码：219 / 225

页数：7

共 40 条

[1]

BEIGHTON D, 1980, MICROBIOS, V27, P117

[2]

BORGARELLI M B, 1985, Revista Argentina de Microbiologia, V17, P41

[3]

BRATTHALL D, 1976, J DENT RES, V55

[4] DNA BASE SEQUENCE HOMOLOGIES AMONG STRAINS OF STREPTOCOCCUS-SANGUIS [J].

COYKENDALL, AL ;

SPECHT, PA .

JOURNAL OF GENERAL MICROBIOLOGY, 1975, 91 (NOV) :92-98

[5] 4 TYPES OF STREPTOCOCCUS-MUTANS BASED ON THEIR GENETIC, ANTIGENIC AND BIOCHEMICAL CHARACTERISTICS [J].

COYKENDALL, AL .

JOURNAL OF GENERAL MICROBIOLOGY, 1974, 83 (AUG) :327-338

[6] 3 EPITOPE-SPECIFIC MONOCLONAL-ANTIBODIES AGAINST THE HAPTEN PENICILLIN [J].

DEHAAN, P ;

DEJONGE, AJR ;

VERBRUGGE, T ;

BOORSMA, DM .

INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY, 1985, 76 (01) :42-46

[7] ACIDOGENESIS BY ORAL STREPTOCOCCI AT DIFFERENT PH VALUES [J].

DESOET, JJ ;

TOORS, FA ;

DEGRAAFF, J .

CARIES RESEARCH, 1989, 23 (01) :14-17

[8] IDENTIFICATION OF STREPTOCOCCUS-SOBRINUS WITH MONOCLONAL-ANTIBODIES [J].

DESOET, JJ ;

VANDALEN, PJ ;

APPELMELK, BJ ;

DEGRAAFF, J .

JOURNAL OF CLINICAL MICROBIOLOGY, 1987, 25 (12) :2285-2288

[9] CHARACTERIZATION OF MONOCLONAL-ANTIBODIES RAISED TO STREPTOCOCCUS-MUTANS [J].

DOYLE, G ;

EVERHART, D .

CURRENT MICROBIOLOGY, 1985, 12 (04) :197-201

[10] PRODUCTION OF MONOCLONAL-ANTIBODY AGAINST A GLUCOSYLTRANSFERASE OF STREPTOCOCCUS-MUTANS 6715 [J].

FURUTA, T ;

NISIZAWA, T ;

CHIBA, J ;

HAMADA, S .

INFECTION AND IMMUNITY, 1983, 41 (02) :872-875

← 1 2 3 4 →