Use of Phytone Peptone to Optimize Growth and Cell Density of Lactobacillus reuteri

被引：14

作者：

Atilola, Olabiyi A. ^{[1
]}

Gyawali, Rabin ^{[1
]}

Aljaloud, Sulaiman O. ^{[2
]}

Ibrahim, Salam A. ^{[1
]}

机构：

[1] Agr & Tech State Univ, Food Microbiol & Biotechnol Lab, 173 Carver Hall, Greensboro, NC 27411 USA

[2] King Saud Univ, Coll Sport Sci & Phys Act, Dept Exercise Physiol, Riyadh 11451, Saudi Arabia

来源：

FOODS | 2015年 / 4卷 / 03期

基金：

美国食品与农业研究所;

关键词：

cell density; growth; L; reuteri; phytone peptone; protein;

D O I：

10.3390/foods4030318

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The objective of this study was to determine the use of phytone peptone to optimize the growth and cell density of Lactobacillus reuteri. Four strains of L. reuteri (DSM 20016, SD 2112, CF 2-7F, and MF 2-3,) were used in this study. An overnight culture of individual strains was inoculated into fresh basal media with various protein sources (peptone, tryptone, proteose peptone #3, phytone peptone, tryptic soy broth, yeast extract, and beef extract). Samples were then mixed well and incubated at 37 degrees C for 15 h. Bacterial growth was monitored by measuring turbidity (optical density 610 nm) at different time intervals during the incubation period. At the end of incubation, samples were plated on de-Man Rogosa Sharpe (MRS) agar to determine the bacterial population. Our results showed that phytone peptone promoted the growth of L. reuteri (p < 0.05) by 1.4 log CFU/mL on average compared to the control samples. Therefore, phytone peptone could be included in laboratory media to enhance growth and increase the cell density of L. reuteri.

引用

页码：318 / 327

页数：10

共 24 条

[1] Carbohydrate and protein sources influence the induction of α- and β-galactosidases in Lactobacillus reuteri [J].

Alazzeh, A. Y. ;

Ibrahim, S. A. ;

Song, D. ;

Shahbazi, A. ;

AbuGhazaleh, A. A. .

FOOD CHEMISTRY, 2009, 117 (04) :654-659

[2] Cell growth and bacteriocin production of probiotic Lactobacillus strains in different media [J].

Avonts, L ;

Van Uytven, E ;

De Vuyst, L .

INTERNATIONAL DAIRY JOURNAL, 2004, 14 (11) :947-955

[3]

DE MAN J. C., 1960, JOUR APPL BACT, V23, P130, DOI 10.1111/j.1365-2672.1960.tb00188.x

[4] Soy protein fortification affects sensory, chemical, and microbiological properties of dairy yogurts [J].

Drake, MA ;

Chen, XQ ;

Tamarapu, S ;

Leenanon, B .

JOURNAL OF FOOD SCIENCE, 2000, 65 (07) :1244-1247

[5]

FULLER R, 1989, J APPL BACTERIOL, V66, P365

[6] The use of crude cellular extracts of Lactobacillus delbrueckii ssp bulgaricus 11842 to stimulate growth of a probiotic Lactobacillus rhamnosus culture in milk [J].

Gaudreau, H ;

Champagne, CP ;

Jelen, P .

ENZYME AND MICROBIAL TECHNOLOGY, 2005, 36 (01) :83-90

[7] Impact of plant derivatives on the growth of foodborne pathogens and the functionality of probiotics [J].

Gyawali, Rabin ;

Ibrahim, Salam A. .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2012, 95 (01) :29-45

[8] Sweet Potatoes as a Basic Component in Developing a Medium for the Cultivation of Lactobacilli [J].

Hayek, Saeed A. ;

Shahbazi, Aboghasem ;

Awaisheh, Saddam S. ;

Shah, Nagendra P. ;

Ibrahim, Salm A. .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2013, 77 (11) :2248-2254

[9]

Ibrahim S. A., 1996, Dairy, Food and Environmental Sanitation, V16, P823

[10] GROWTH-PROMOTING FACTORS FOR BIFIDOBACTERIUM-LONGUM [J].

IBRAHIM, SA ;

BEZKOROVAINY, A .

JOURNAL OF FOOD SCIENCE, 1994, 59 (01) :189-191

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