RERECOVERY OF 14C-LABELED SUGAR AND ALCOHOL DERIVATIVES IN GAS CHROMATOGRAPHY

The recovery of trimethylsilyl (TMS) derivatives in gas chromatography was determined by using 14C-labeled compounds. Under the conditions used approximately one-half of the cholesterol derivative was recovered. Four-fifths of the glycerol derivative and one-fourth of the stearyl alcohol derivative were recovered. Approximately one-fourth of the TMS derivatives of glucose, fructose and sucrose were recovered. Rechromatography of the TMS derivative of glucose resulted in the same loss as found initially. With the TMS derivative of glocuse-14C, radioactivity was found throughout the length of the column. Thus decomposition and/or deposition of the derivative occurred throughout the column. The recovery of the triflouroacetyl derivative of glucose was less than of the TMS derivative. © 1968.