2-DIMENSIONAL CRYSTALLIZATION OF AVIDIN ON BIOTINYLATED LIPID MONOLAYERS

被引：18
|
作者：
QIN, H [1 ]
LIU, Z [1 ]
SUI, SF [1 ]
机构：
[1] TSING HUA UNIV, DEPT BIOL SCI & BIOTECHNOL, BIOPHYS LAB, BEIJING 100084, PEOPLES R CHINA
来源：
BIOPHYSICAL JOURNAL | 1995年 / 68卷 / 06期
关键词：
D O I：
10.1016/S0006-3495(95)80431-5
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
Two-dimensional crystals of avidin were obtained on mixed lipid monolayers containing biotinylated lipids (N-biotinyl-dipalmitolyl-L-alpha-phosphatidyl ethanolamine and dioleoyl phosphatidyl choline) by specific interaction. Image analysis of electron micrographs of these crystals revealed p2 symmetry with the unit cell parameters a = 66 +/- 2 Angstrom, b = 68 +/- 1 Angstrom, and gamma = 121 +/- 4 degrees. The projection map showed, at a resolution of about 27 Angstrom, that the four subunits within one avidin molecule are separated into two parts. Comparison between avidin and streptavidin reveals that avidin molecule binds to the lipid monolayer in an orientation similar to that of streptavidin.
引用
收藏
页码：2493 / 2496
页数：4
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