HIGH-YIELD ISOLATION AND EFFECT ON PROTON LEAKAGE OF GLYCEOLLIN-I AND GLYCEOLLIN-III

被引：2

作者：

GIANNINI, JL

HALVORSON, JS

SPESSARD, GO

机构：

[1] ST OLAF COLL, DEPT BIOL, 1520 ST OLAF AVE, NORTHFIELD, MN 55057 USA

[2] ST OLAF COLL, DEPT CHEM, NORTHFIELD, MN 55057 USA

来源：

PHYTOCHEMISTRY | 1991年 / 30卷 / 10期

关键词：

GLYCINE-MAX; LEGUMINOSAE; SOYBEAN; GLYCEOLLIN; H+ LEAKAGE;

D O I：

10.1016/0031-9422(91)83182-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple, high-yield method for the isolation of the pterocarpan phytoalexins glyceollin I and III has been developed. Isolation and purification were achieved using a combination of silica gel column and preparative radial chromatography. Isomers I and III were purified to > 95% as shown by HPLC. Both caused proton leakage from sealed red beet tonoplast vesicles and isolated tonoplast vacuoles. Glyceollin III was more active than glyceollin I in causing proton leakage.

引用

页码：3233 / 3236

页数：4