The influence of corneal stromal matrix proteins on the migration of human corneal fibroblasts

被引:39
|
作者
Andresen, JL [1 ]
Ledet, T
Hager, H
Josephsen, K
Ehlers, N
机构
[1] Aarhus Univ Hosp, Dept Ophthalmol, DK-8000 Aarhus C, Denmark
[2] Aarhus Univ Hosp, Res Lab Biochem Pathol, DK-8000 Aarhus C, Denmark
[3] Univ Aarhus, Royal Dent Coll, Fac Hlth Sci, Dept Biochem, DK-8000 Aarhus C, Denmark
[4] Univ Aarhus, Royal Dent Coll, Fac Hlth Sci, Dept Dent Pathol Operat Dent & Endodont, DK-8000 Aarhus C, Denmark
关键词
keratocytes; corneal fibroblasts; cell migration; cell-matrix interactions; extracellular matrix; cell adhesion; integrins;
D O I
10.1006/exer.2000.0850
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
Motivated by the alterations seen in the corneal matrix composition after photorefractive keratectomy and the migration of corneal keratocytes seen following this procedure. the locomotor response of corneal stromal fibroblasts to various extracellular matrix proteins was determined. In addition. the involvement of integrin mediated attachment to the matrix proteins was investigated. Quantitative invasion assays were performed using collagen gels, supplemented with either fibronectin, tenascin, collagen type V, collagen type VI, chondroitin sulfate or keratan sulfate, The ultrastructure of the gels was visualized by scanning electron microscopy and related to the migration results. The extent of alpha(1)beta(1), alpha(2)beta(1), alpha(3)beta(1) and alpha(5)beta(1) integrin mediated attachment to the matrix proteins was evaluated using blocking antibodies. Fibronectin increased corneal fibroblast migration significantly, and served as an excellent substrate for cellular attachment, mediated by the alpha(5)beta(1) integrin. Addition of tenascin to the fibronectin-containing gels disrupted these effects, while attachment to this matrix also involved the integrins alpha(2)beta(1) and alpha(3)beta(1) Chondroitin sulfate and collagen types V and VI primarily altered the structure of the collagen matrix, resulting in an inhibition of migration by the collagens and an increase by chondroitin sulfate. They all served as poor substrates for attachment. Thus, the migratory activity of corneal fibroblasts in vitro is influenced by the composition of the surrounding extracellular matrix, either by integrin mediated cell-matrix interactions or through matrix-matrix interactions. This study provides evidence that the provisional matrix deposited in a corneal stromal wound may facilitate the entry of migrating corneal fibroblasts. (C) 2000 Academic Press.
引用
收藏
页码:33 / 43
页数:11
相关论文
共 50 条
  • [1] Nerve regeneration by human corneal stromal keratocytes and stromal fibroblasts
    Yam, Gary Hin-Fai
    Williams, Geraint P.
    Setiawan, Melina
    Yusoff, Nur Zahirah Binte M.
    Lee, Xiao-wen
    Htoon, Hla Myint
    Zhou, Lei
    Fuest, Matthias
    Mehta, Jodhbir S.
    SCIENTIFIC REPORTS, 2017, 7
  • [2] Nerve regeneration by human corneal stromal keratocytes and stromal fibroblasts
    Gary Hin-Fai Yam
    Geraint P. Williams
    Melina Setiawan
    Nur Zahirah Binte M. Yusoff
    Xiao-wen Lee
    Hla Myint Htoon
    Lei Zhou
    Matthias Fuest
    Jodhbir S. Mehta
    Scientific Reports, 7
  • [3] BIOSYNTHESIS OF STROMAL MATRIX PROTEOGLYCANS AND BASEMENT-MEMBRANE COMPONENTS BY HUMAN CORNEAL FIBROBLASTS
    HASSELL, JR
    SCHRECENGOST, PK
    RADA, JA
    SUNDARRAJ, N
    SOSSI, G
    THOFT, RA
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 1992, 33 (03) : 547 - 557
  • [4] Corneal stromal stem cells versus corneal fibroblasts in generating structurally appropriate corneal stromal tissue
    Wu, Jian
    Du, Yiqin
    Mann, Mary M.
    Funderburgh, James L.
    Wagner, William R.
    EXPERIMENTAL EYE RESEARCH, 2014, 120 : 71 - 81
  • [6] Effect of tenascin and fibronectin on the migration of human corneal fibroblasts
    Schmidinger, G
    Hanselmayer, G
    Pieh, S
    Lackner, B
    Kaminski, S
    Ruhswurm, I
    Skorpik, C
    JOURNAL OF CATARACT AND REFRACTIVE SURGERY, 2003, 29 (02): : 354 - 360
  • [7] Transcriptomic landscape of quiescent and proliferating human corneal stromal fibroblasts
    Kumar, Rajnish
    Tripathi, Ratnakar
    Sinha, Nishant R.
    Mohan, Rajiv R.
    EXPERIMENTAL EYE RESEARCH, 2024, 248
  • [8] Characterization of hydrogen sulfide toxicity to human corneal stromal fibroblasts
    Balne, Praveen K.
    Sinha, Nishant R.
    Hofmann, Alexandria C.
    Martin, Lynn M.
    Mohan, Rajiv R.
    ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 2020, 1480 (01) : 207 - 218
  • [9] Nerve regeneration by human corneal stromal keratocytes (CSKs) and stromal fibroblasts (SFs)
    Yam, Gary Hin-Fai
    Williams, Geraint P.
    Fuest, Matthias
    Lee, Xiao-Wen
    Zhou, Lei
    Mehta, Jodhbir
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2017, 58 (08)
  • [10] Influence of Extracellular Matrix Proteins and Substratum Topography on Corneal Epithelial Cell Alignment and Migration
    Raghunathan, VijayKrishna
    McKee, Clayton
    Cheung, Wai
    Naik, Rachel
    Nealey, Paul F.
    Russell, Paul
    Murphy, Christopher J.
    TISSUE ENGINEERING PART A, 2013, 19 (15-16) : 1713 - 1722