ROLE OF CA2+ IN ALLOXAN-INDUCED PANCREATIC BETA-CELL DAMAGE

被引：53

作者：

KIM, HR ^{[1
]}

RHO, HW ^{[1
]}

PARK, BH ^{[1
]}

PARK, JW ^{[1
]}

KIM, JS ^{[1
]}

KIM, UH ^{[1
]}

CHUNG, MY ^{[1
]}

机构：

[1] CHONNAM NATL UNIV,SCH MED,DEPT INTERNAL MED,KWANGJU,SOUTH KOREA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE | 1994年 / 1227卷 / 1-2期

关键词：

ALLOXAN; CALCIUM; DIABETES MELLITUS; HYDROGEN PEROXIDE; VERAPAMIL;

D O I：

10.1016/0925-4439(94)90111-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pretreatment of rats with verapamil, a Ca2+-antagonist, completely prevented alloxan-induced hyperglycemia. Verapamil also abolished the inhibition of insulin secretion by alloxan and H2O2 in isolated rat pancreatic islets. H2O2 generation from alloxan was not affected by verapamil, but alloxan- and H2O2-induced DNA strand breaks were completely prevented. Treatment of beta-cells with alloxan and H2O2 caused elevation of cytosolic free Ca2+, and this increase of Ca2+ was also abolished by verapamil. These results suggest that alloxan-derived oxygen radicals may disturb intracellular Ca2+ homeostasis by increasing Ca2+ influx, which results in secondary reactions ultimately leading to DNA strand breaks and cytotoxicity of beta-cells.

引用

页码：87 / 91

页数：5

共 31 条

[1] ARENDS MJ, 1990, AM J PATHOL, V136, P593
[2] UPTAKE OF LABELED ALLOXAN IN MOUSE ORGANS AND MITOCHONDRIA INVIVO AND INVITRO
BOQUIST, L
NELSON, L
LORENTZON, R
[J]. ENDOCRINOLOGY, 1983, 113 (03) : 943 - 948
[3] FREE-RADICALS ALTER IONIC CALCIUM LEVELS AND MEMBRANE PHOSPHOLIPIDS IN CULTURED RAT VENTRICULAR MYOCYTES
BURTON, KP
MORRIS, AC
MASSEY, KD
BUJA, LM
HAGLER, HK
[J]. JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 1990, 22 (09) : 1035 - 1047
[4] COHEN G, 1974, J BIOL CHEM, V249, P2447
[5] COLCA JR, 1983, J BIOL CHEM, V258, P7260
[6] COOPERSTEIN SJ, 1978, J PHARMACOL EXP THER, V204, P230
[7] Dunn JS, 1943, LANCET, V1, P484
[8] INHIBITION OF ALLOXAN ACTION IN ISOLATED PANCREATIC-ISLETS BY SUPEROXIDE-DISMUTASE, CATALASE, AND A METAL CHELATOR
FISCHER, LJ
HAMBURGER, SA
[J]. DIABETES, 1980, 29 (03) : 213 - 216
[9] SPECIFIC DETERMINATION OF BLOOD GLUCOSE WITH O-TOLUIDINE
HYVARINEN, A
NIKKILA, EA
[J]. CLINICA CHIMICA ACTA, 1962, 7 (01) : 140 - &
[10] JONES DP, 1989, J BIOL CHEM, V264, P6398

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