A SIMPLE FIELD METHOD FOR THE PURIFICATION OF ONCHOCERCA-OCHENGI MICROFILARIAE FROM A MIXED ONCHOCERCA INFECTION IN CATTLE

被引：0

作者：

HAGEN, HE

KLAGER, SL

HAM, PJ

机构：

来源：

TROPICAL MEDICINE AND PARASITOLOGY | 1995年 / 46卷 / 03期

基金：

英国惠康基金;

关键词：

D O I：

暂无

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Onchocerca ochengi, a bovine parasite, is a suitable model for research on human River Blindness. However, the microfilariae are normally found concomitantly with at least one of the other three bovine Onchocerca species O. dukei, O. gutturosa and O. armillata causing difficulties for the work on the microfilariae. We describe a simple and field applicable method for the separation of living O. ochengi microfilariae from the other Onchocerca species using Sephadex G-25 columns. Elution of mixed populations resulted in the passage of O. gutturosa and/or O. dukei in the initial 1 mi fraction with O. ochengi eluting as an almost 100% pure species in the 4th and 5th fractions.

引用

页码：201 / 202

页数：2

共 9 条

[1]

[Anonymous], 2011, USE R

[2]

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