FLOW-INJECTION DETERMINATION OF L-GLUTAMATE IN SERUM WITH AN IMMOBILIZED GLUTAMATE-DEHYDROGENASE REACTOR

被引：10
|
作者：
KIBA, N
MORIYA, T
FURUSAWA, M
机构：
[1] Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University, Kofu
来源：
ANALYTICA CHIMICA ACTA | 1992年 / 256卷 / 02期
关键词：
FLOW SYSTEM; FLUOROMETRY; ENZYME REACTOR; GLUTAMATE; SERUM;
D O I：
10.1016/0003-2670(92)85346-8
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Glutamate dehydrogenase is immobilized on poly(vinyl alcohol) beads and packed into a stainless-steel column (3 cm X 4 mm i.d.). Serum is deproteinized with tungstic acid. Sample solution (30-mu-l) is injected into the carrier stream [5 mM NAD+ in glycine buffer (pH 9.5)]. The NADH formed is detected at 465 nm (excitation at 340 nm). The calibration graph is linear for 0.5 - 500-mu-M glutamate; the detection limit is 0.2-mu-M.
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页码：221 / 224
页数：4
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