SOLID-PHASE SYNTHESIS AND SOME PHARMACOLOGICAL PROPERTIES OF [8-PHENYLALANINE]-OXYTOCIN

被引：27
作者：
BAXTER, JWM
MANNING, M
SAWYER, WH
机构：
[1] Department of Biochemistry, McGill University, Montreal, Quebec
[2] Department of Biochemistry, Medical College of Ohio, Toledo
[3] Department of Pharmacology, College of Physicians and Surgeons of Columbia University
来源：
BIOCHEMISTRY | 1969年 / 8卷 / 09期
关键词：
D O I：
10.1021/bi00837a015
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In an attempt to establish the identity of a principle designated as EOF I (elasmobranch oxytocin-like principle I), which has been isolated from the pituitary gland of the spiny dogfish (Squalus acanthias), [8-phenylalanine]-oxytocin has been synthesized and tested for some of the pharmacological activities characteristic of the neurohypophysial hormones. The synthesis was accomplished by means of the Merrifield solid-phase method as employed for the synthesis of oxytocin and glumitocin. After purification by gel filtration on Sephadex G-15, the product was obtained in 38% over-all yield. Bioassays yielded the following activities with their 95% confidence limits: rat uterus (no Mg2+), 108 (100-117) units/mg; rat uterus (+Mg2+), 366 (332-406) units/mg; fowl vasodepressor, 191 (170-214) units/mg; rabbit milk ejection, 341 (316-368) units/mg; rat vasopressor, 0.97 (0.90-1.04) units/ mg; rat antidiuretic, 1.16 (0.92-1.46) units/mg; and frog bladder activity, 1700 (1240-2360) units/mg. A comparison of these properties with those obtained for EOP I indicates that the latter is not [8-phenylalanine]-oxytocin. © 1969, American Chemical Society. All rights reserved.
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页码：3592 / &
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