Four new species of spiders belonging to the genus Dysdera Walk., 1937 from Middle Asia are described: D. kugitangica Dunin, sp.n. from Turkmenistan (Kugitang), D. kronebergi Dunin, sp.n. and D. fedtschenkoi Dunin sp.n. from the Southern Tadjikistan, D. pamirica Dunin sp.n. from Central Tadjikistan (Peter the Great Ridge).