PROTHORACICOTROPIC HORMONE LIKE ACTIVITY IN THE EMBRYONATED EGGS OF GYPSY-MOTH, LYMANTRIA-DISPAR (L)

被引：11

作者：

MASLER, EP ^{[1
]}

BELL, RA ^{[1
]}

THYAGARAJA, BS ^{[1
]}

KELLY, TJ ^{[1
]}

BORKOVEC, AB ^{[1
]}

机构：

[1] UNIV MARYLAND,DEPT ZOOL,COLLEGE PK,MD 20705

来源：

JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY | 1991年 / 161卷 / 01期

关键词：

EMBRYONIC; GYPSY MOTH; INVITRO BIOASSAY; PROTEIN CHARACTERIZATION; PROTHORACICOTROPIC HORMONE;

D O I：

10.1007/BF00258744

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

Prothoracicotropic hormone (PTTH)-like activity was obtained from embryonated eggs of the gypsy moth, Lymantria dispar. Activity was detected using an in vitro prothoracic gland stimulation bioassay. Dose-response kinetics of crude extract revealed a 4-fold activation range with a maximum activation of 35-fold. Nearly 70% of the activity was sensitive to denaturation by heat or organic solvent extraction. Heat and organic solvent-stable activity is due to a protein. Dose-response kinetics suggest the presence of a small molecular weight PTTH with pre-hatch eggs providing a rich source of the hormone.

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页码：37 / 41

页数：5

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