PROTHORACICOTROPIC HORMONE LIKE ACTIVITY IN THE EMBRYONATED EGGS OF GYPSY-MOTH, LYMANTRIA-DISPAR (L)

被引:11
作者
MASLER, EP [1 ]
BELL, RA [1 ]
THYAGARAJA, BS [1 ]
KELLY, TJ [1 ]
BORKOVEC, AB [1 ]
机构
[1] UNIV MARYLAND,DEPT ZOOL,COLLEGE PK,MD 20705
关键词
EMBRYONIC; GYPSY MOTH; INVITRO BIOASSAY; PROTEIN CHARACTERIZATION; PROTHORACICOTROPIC HORMONE;
D O I
10.1007/BF00258744
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Prothoracicotropic hormone (PTTH)-like activity was obtained from embryonated eggs of the gypsy moth, Lymantria dispar. Activity was detected using an in vitro prothoracic gland stimulation bioassay. Dose-response kinetics of crude extract revealed a 4-fold activation range with a maximum activation of 35-fold. Nearly 70% of the activity was sensitive to denaturation by heat or organic solvent extraction. Heat and organic solvent-stable activity is due to a protein. Dose-response kinetics suggest the presence of a small molecular weight PTTH with pre-hatch eggs providing a rich source of the hormone.
引用
收藏
页码:37 / 41
页数:5
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