EMBRYONIC;
GYPSY MOTH;
INVITRO BIOASSAY;
PROTEIN CHARACTERIZATION;
PROTHORACICOTROPIC HORMONE;
D O I:
10.1007/BF00258744
中图分类号:
Q4 [生理学];
学科分类号:
071003 ;
摘要:
Prothoracicotropic hormone (PTTH)-like activity was obtained from embryonated eggs of the gypsy moth, Lymantria dispar. Activity was detected using an in vitro prothoracic gland stimulation bioassay. Dose-response kinetics of crude extract revealed a 4-fold activation range with a maximum activation of 35-fold. Nearly 70% of the activity was sensitive to denaturation by heat or organic solvent extraction. Heat and organic solvent-stable activity is due to a protein. Dose-response kinetics suggest the presence of a small molecular weight PTTH with pre-hatch eggs providing a rich source of the hormone.
机构:Insect Neurobiology and Hormone Laboratory, Plant Sciences Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, 20705-2350, MD
MASLER, EP
RAINA, AK
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机构:Insect Neurobiology and Hormone Laboratory, Plant Sciences Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, 20705-2350, MD