A LIPOPROTEIN INDEPENDENT ASSAY FOR HUMAN-SERUM LECITHIN-CHOLESTEROL ACYLTRANSFERASE

被引：8

作者：

MORIN, RJ ^{[1
]}

PIRAN, U ^{[1
]}

机构：

[1] UNIV CALIF LOS ANGELES, HARBOR GEN HOSP, MED CTR, DEPT PATHOL, TORRANCE, CA 90509 USA

来源：

CLINICA CHIMICA ACTA | 1981年 / 111卷 / 2-3期

关键词：

D O I：

10.1016/0009-8981(81)90188-1

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A method was developed for estimation of human serum lecithin-cholesterol acyltransferase free of interference by endogenous lipoproteins. Precipitation of serum low and very low density lipoproteins by sodium phosphotungstate and MgCl2 results in complete recovery of lecithin-cholesterol acyltransferase activity in the supernatant. One .mu.l of the supernatant can be accurately assayed with a highly efficient substrate containing phosphatidylcholine-cholesterol vesicles and apo-high density lipoproteins (HDL), with no inferference from endogenous HDL or residual precipitation reagents. Serum levels of the enzyme were reduced in patients with parenchymal liver disease, renal disease, gastrointestinal tumors and anemias.

引用

页码：211 / 218

页数：8

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