CLONING, CHARACTERIZATION, AND EXPRESSION OF A CDNA-ENCODING AN INDUCIBLE NITRIC-OXIDE SYNTHASE FROM THE HUMAN CHONDROCYTE

被引：291

作者：

CHARLES, IG ^{[1
]}

PALMER, RMJ ^{[1
]}

HICKERY, MS ^{[1
]}

BAYLISS, MT ^{[1
]}

CHUBB, AP ^{[1
]}

HALL, VS ^{[1
]}

MOSS, DW ^{[1
]}

MONCADA, S ^{[1
]}

机构：

[1] KENNEDY INST, LONDON W6 7DW, ENGLAND

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1993年 / 90卷 / 23期

关键词：

D O I：

10.1073/pnas.90.23.11419

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Incubation of human articular chondrocytes with interleukin 1beta results in the time-dependent expression of nitric oxide (NO) synthase. We report here the isolation of a cDNA clone which encodes a protein of 1153 amino acids with a molecular mass of 131,213 Da and a calculated isoelectric point of 7.9. CHO cells transfected with a plasmid harboring this cDNA clone expressed NO synthase activity that was inhibited by some L-arginine analogues. The deduced amino acid sequence of the human chondrocyte inducible NO synthase shows 51% identity and 68% similarity with the endothelial NO synthase and 54% identity and 70% similarity with the neuronal NO synthase. The similarity (88%) between the human chondrocyte NO synthase cDNA sequence and that reported for the murine macrophage suggests that the inducible class of enzyme is conserved between different cell types and across species.

引用

页码：11419 / 11423

页数：5

共 53 条

[1] NG-METHYLARGININE, AN INHIBITOR OF ENDOTHELIUM-DERIVED NITRIC-OXIDE SYNTHESIS, IS A POTENT PRESSOR AGENT IN THE GUINEA-PIG - DOES NITRIC-OXIDE REGULATE BLOOD-PRESSURE INVIVO [J].