EXPRESSION CLONING OF AN ADENYLATE-CYCLASE COUPLED CALCITONIN RECEPTOR

被引：420

作者：

LIN, HY

HARRIS, TL

FLANNERY, MS

ARUFFO, A

KAJI, EH

GORN, A

KOLAKOWSKI, LF

LODISH, HF

GOLDRING, SR

机构：

[1] WHITEHEAD INST BIOMED RES, CAMBRIDGE, MA 02142 USA

[2] MIT, DEPT BIOL, CAMBRIDGE, MA 02139 USA

[3] HARVARD UNIV, SCH MED, DEPT MED, BOSTON, MA 02115 USA

[4] MASSACHUSETTS GEN HOSP, MED SERV, ARTHRIT UNIT, BOSTON, MA 02114 USA

[5] NEW ENGLAND DEACONESS HOSP, BOSTON, MA 02215 USA

来源：

SCIENCE | 1991年 / 254卷 / 5034期

关键词：

D O I：

10.1126/science.1658940

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A calcitonin receptor complementary DNA (cDNA) was cloned by expression of a cDNA library from a porcine kidney epithelial cell line in COS cells. The 482-amino acid receptor has high affinity for salmon calcitonin (dissociation constant K(d) almost-equal-to 6 nM) and is functionally coupled to increases in intracellular cyclic adenosine monophosphate (cAMP). The receptor shows no sequence similarity to other reported G protein-coupled receptors but is homologous to the parathyroid hormone-parathyroid hormone-related peptide (PTH-PTHrP) receptor, indicating that the receptors for these hormones, which regulate calcium homeostasis, represent a new family of G protein-coupled receptors.

引用

页码：1022 / 1024

页数：3

共 19 条

[1] MULTIPLE SEQUENCE ALIGNMENT OF PROTEIN FAMILIES SHOWING LOW SEQUENCE HOMOLOGY - A METHODOLOGICAL APPROACH USING DATABASE PATTERN-MATCHING DISCRIMINATORS FOR G-PROTEIN-LINKED RECEPTORS [J].