21-PERCENT PARTIAL-HEPATECTOMY - INVIVO RAT MODEL FOR THE STUDY OF LIVER-REGENERATION

被引：2

作者：

BARUCH, Y

FRITH, LO

KIRSCH, RE

机构：

[1] TECHNION ISRAEL INST TECHNOL,FAC MED,IL-31096 HAIFA,ISRAEL

[2] UNIV CAPE TOWN,SCH MED,OBSERV,CAPE TOWN,SOUTH AFRICA

来源：

JOURNAL OF HEPATOLOGY | 1991年 / 12卷 / 03期

关键词：

D O I：

10.1016/0168-8278(91)90833-W

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

We describe a new model of submaximal stimulation of liver regeneration in the rat. Removal of the right lateral lobes in the rat produced a 21% hepatectomy. The measurement accuracy of the incorporation rate of [C-14]thymidine into DNA 24 h after 21% hepatectomy (n = 32) was less than that after the standard 34% hepatectomy (n = 32), with S.D./mean being 35% and 130%, respectively (F-test, p < 0.025). This model was able to detect regeneration-stimulation activity present in liver cytosol extract and serum of 68% hepatectomized rats. Using this model we identified a subfraction of rat serum achieved after treatment of serum with ethanol and ion-exchange column, which had a highly stimulatory effect. Stimulation obtained with serum from hepatectomized pigs showed that these factors are not species specific.

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页码：316 / 320

页数：5

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