MEASUREMENT OF CELLULOLYTIC ACTIVITY BY LOW-PRESSURE LIQUID-CHROMATOGRAPHY

被引：9
作者：
LADISCH, MR
ANDERSON, AW
TSAO, GT
机构：
[1] Laboratory of Renewable Resources Enqineerina, Purdue University, West Lafayette
来源：
JOURNAL OF LIQUID CHROMATOGRAPHY | 1979年 / 2卷 / 05期
基金：
美国国家科学基金会;
关键词：
D O I：
10.1080/01483917908060100
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The application of aqueous, low pressure liquid chromatography to the assay of cellulolytic enzyme activity is discussed. The advantages of this method are speed of analysis (less than 20 min.), small sample size (20 ka), Good resolution, and a tolerance of the system to the presence of extraneous salts and proteins. Examples showing the use of this tool are given. © 1979, Taylor & Francis Group, LLC. All rights reserved.
引用
收藏
页码：745 / 760
页数：16
相关论文
共 17 条
[1]   SITE OF HYDROLYSIS OF CELLULODEXTRINS + REDUCED CELLULODEXTRINS BY PURIFIED CELLULASE COMPONENTS [J].
COLE, FE ;
KING, KW .
BIOCHIMICA ET BIOPHYSICA ACTA, 1964, 81 (01) :122-&
[2]  
CONRAD EC, 1976, FOOD TECHNOL, V84
[3]   COLORIMETRIC METHOD FOR DETERMINATION OF SUGARS AND RELATED SUBSTANCES [J].
DUBOIS, M ;
GILLES, KA ;
HAMILTON, JK ;
REBERS, PA ;
SMITH, F .
ANALYTICAL CHEMISTRY, 1956, 28 (03) :350-356
[4]  
GONG CS, ADV CHEM SERIES
[5]   SEPARATION OF OLIGOSACCHARIDES BY PARTITION CHROMATOGRAPHY ON ION-EXCHANGE RESINS [J].
HAVLICEK, J ;
SAMUELSON, O .
ANALYTICAL CHEMISTRY, 1975, 47 (11) :1854-1857
[6]  
HUEBNER A, BIOTECHNOL BIOENG
[7]   HIGH-SPEED LIQUID-CHROMATOGRAPHY OF CELLODEXTRINS AND OTHER SACCHARIDE MIXTURES USING WATER AS ELUENT [J].
LADISCH, MR ;
HUEBNER, AL ;
TSAO, GT .
JOURNAL OF CHROMATOGRAPHY, 1978, 147 (JAN) :185-193
[8]  
LADISCH MR, J CHROMATOGRAPHY
[9]  
MANDELS M, 1976, BIOTECHNOL BIOENG, P21
[10]   PARTITION CHROMATOGRAPHY OF SUGARS ON ION-EXCHANGE RESINS [J].
MARTINSSON, E ;
SAMUELSON, O .
JOURNAL OF CHROMATOGRAPHY, 1970, 50 (03) :429-+
12