IMPACT OF ANTIGEN-PRESENTING CELLS ON CYTOKINE PROFILES OF HUMAN TH CLONES ESTABLISHED AFTER STIMULATION WITH MYCOBACTERIUM-TUBERCULOSIS ANTIGENS

被引：17
作者：
CONRADT, P
KAUFMANN, SHE
机构：
来源：
INFECTION AND IMMUNITY | 1995年 / 63卷 / 05期
关键词：
D O I：
10.1128/IAI.63.5.2079-2081.1995
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Human T cells reactive with mycobacterial antigens are generally considered to correlate with a Th1 cytokine profile. Our data show that, in addition, Th0 and Th2 clones develop in bulk culture with appropriate antigen-presenting cells before cloning. CD4(+) blasts activated by mycobacterial antigens were cloned, and their mRNA patterns for the interleukins (IL) IL-2, IL-4, IL-5, IL-6, and IL-10 and gamma interferon were characterized by reverse-transcribed PCR. Nonadherent, nonrosetting, enriched peripheral blood mononuclear cells promoted development of Th0; after further depletion of monocytes and natural killer cells? Th2 clones were also found, Epstein-Barr virus-transformed B cells, with specificity for the stimulating antigen, increased the proportion of Th2 clones.
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页码：2079 / 2081
页数：3
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