STABLE TRANSFECTION OF THE NR1 SUBUNIT IN CHINESE-HAMSTER OVARY CELLS FAILS TO PRODUCE A FUNCTIONAL N-METHYL-D-ASPARTATE RECEPTOR

被引：32

作者：

BOECKMAN, FA ^{[1
]}

AIZENMAN, E ^{[1
]}

机构：

[1] UNIV PITTSBURGH,SCH MED,DEPT NEUROBIOL,PITTSBURGH,PA 15261

来源：

NEUROSCIENCE LETTERS | 1994年 / 173卷 / 1-2期

关键词：

NMDAR1; NMDAR2A; CGS-19755; CHO-K1; PATCH-CLAMPING; TRANSFECTION;

D O I：

10.1016/0304-3940(94)90180-5

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The NR1 subunit of the N-methyl-D-aspartate (NMDA) receptor cDNA was stably transfected into Chinese hamster ovary (CHO) cells. Northern analysis revealed 3 clonal cell lines expressing high levels of NMDAR1 mRNA (BA1, BA2 and BA3). NMDAR1 protein was readily detected by Western analysis in only one of the clonal populations, BA1. Whole-cell patch clamp analysis revealed a lack of functional NMDA receptors in all of the cell lines, including BA1. NMDA-mediated responses were obtained only after a second subunit, NMDAR2A, was transiently expressed in BAI cells. Therefore, the NR1 subunit alone may not be sufficient to form functional NMDA receptors in CHO cells.

引用

页码：189 / 192

页数：4

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