ENHANCEMENT OF MURINE MACROPHAGE BINDING OF AND RESPONSE TO BACTERIAL LIPOPOLYSACCHARIDE (LPS) BY LPS-BINDING PROTEIN

被引:48
作者
CORRADIN, SB
MAUEL, J
GALLAY, P
HEUMANN, D
ULEVITCH, RJ
TOBIAS, PS
机构
[1] CHU VAUDOIS, DIV INFECT DIS, CH-1011 LAUSANNE, SWITZERLAND
[2] SCRIPPS CLIN & RES FDN, DEPT IMMUNOL, LA JOLLA, CA 92037 USA
关键词
LEISHMANIA; NITRIC OXIDE; NITRITE; TUMOR NECROSIS FACTOR;
D O I
10.1002/jlb.52.4.363
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We have studied the effects of highly purified rabbit lipopolysaccharide (LPS)-binding protein (LBP) on the ability of murine bone marrow-derived macrophages to respond to bacterial LPS. Macrophage responses studied include the secretion of tumor necrosis factor alpha, production of arginine-derived nitrite (NO2-), and killing of an intracellular pathogen, Leishmania enriettii. Macrophages from either CBA or LPS-hyporesponsive C3H/HeJ mice exhibited significantly greater sensitivity to LPS in the presence of LBP. Furthermore, both CBA and C3H/Hej macrophages demonstrated an LBP-dependent enhancement of LPS binding. These results suggest that C3H/Hej macrophages are capable of binding LPS-LBP complexes and support the hypothesis that hyporesponsiveness in this strain involves a step subsequent to LPS binding. Furthermore, these findings provide additional evidence of the important role played by the acute-phase plasma protein LBP in modifying host response to LPS.
引用
收藏
页码:363 / 368
页数:6
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