BIFUNCTIONAL LYSOZYME GALACTOMANNAN CONJUGATE HAVING EXCELLENT EMULSIFYING PROPERTIES AND BACTERICIDAL EFFECT

被引:109
作者
NAKAMURA, S
KATO, A
KOBAYASHI, K
机构
[1] YAMAGUCHI UNIV,DEPT BIOCHEM,YAMAGUCHI 753,JAPAN
[2] UBE COLL,DEPT FOOD & NUTR,YAMAGUCHI 755,JAPAN
关键词
D O I
10.1021/jf00017a005
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Lysozyme-galactomannan conjugate prepared through controlled Maillard reaction revealed excellent emulsifying properties and antimicrobial properties. The lytic activity of the conjugate remained about 80% that of native lysozyme when measured by using Micrococcus lysodeikticus as a substrate. The emulsifying properties of the conjugate were superior to those of commercial emulsifiers. The emulsifying activity and stability of the lysozyme-galactomannan conjugate were not affected in the presence of 0.2 M NaCl and in acidic pH, while those of commercial emulsifiers were decreased. In addition, the lysozyme-galactomannan conjugate exhibited a lethal antimicrobial effect against Gram-negative bacteria.
引用
收藏
页码:735 / 739
页数:5
相关论文
共 8 条
[1]   MODIFICATION OF AMINO GROUPS IN INHIBITORS OF PROTEOLYTIC ENZYMES [J].
HAYNES, R ;
OSUGA, DT ;
FEENEY, RE .
BIOCHEMISTRY, 1967, 6 (02) :541-&
[2]   EMULSIFYING PROPERTIES OF PROTEIN POLYSACCHARIDE COMPLEXES AND HYBRIDS [J].
KATO, A ;
SATO, T ;
KOBAYASHI, K .
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1989, 53 (08) :2147-2152
[3]   FUNCTIONAL PROTEIN POLYSACCHARIDE CONJUGATE PREPARED BY CONTROLLED DRY-HEATING OF OVALBUMIN DEXTRAN MIXTURES [J].
KATO, A ;
SASAKI, Y ;
FURUTA, R ;
KOBAYASHI, K .
AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1990, 54 (01) :107-112
[4]   PREPARATION AND CHARACTERIZATION OF OVALBUMIN-DEXTRAN CONJUGATE HAVING EXCELLENT EMULSIFYING PROPERTIES [J].
KATO, A ;
MURATA, K ;
KOBAYASHI, K .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1988, 36 (03) :421-425
[5]   CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].
LAEMMLI, UK .
NATURE, 1970, 227 (5259) :680-+
[6]   NEW ANTIMICROBIAL CHARACTERISTICS OF LYSOZYME DEXTRAN CONJUGATE [J].
NAKAMURA, S ;
KATO, A ;
KOBAYASHI, K .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1991, 39 (04) :647-650
[7]   EMULSIFYING PROPERTIES OF PROTEINS - EVALUATION OF A TURBIDIMETRIC TECHNIQUE [J].
PEARCE, KN ;
KINSELLA, JE .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1978, 26 (03) :716-723
[8]  
YAMAMOTO T, 1990, DENPUN KAGAKU, V37, P99