MICROBIOLOGICAL CHARACTERIZATION OF A FUEL-OIL CONTAMINATED SITE INCLUDING NUMERICAL IDENTIFICATION OF HETEROTROPHIC WATER AND SOIL BACTERIA

Seven soil samples and seven groundwater samples from a site contaminated with fuel-oil were investigated using several chemical and microbiological techniques. In soil samples, 500 to 7,500 mg/kg of total hydrocarbons were found. These samples contained no n-alkanes but iso-and branched chain alkanes. No polychlorinated biphenyls could be detected. Microbiological investigations included estimations of total cell counts, viable cell counts on different media, and numbers of methylotrophic, denitrifying, sulphate reducing, anaerobic (with the exception of methanogenic organisms), and hydrocarbon degrading bacteria. Viable and hydrocarbon degrading bacteria were found in all samples. A total of 1,366 pure cultures was characterized morphologically and physiologically and identified by numerical identification using a data base of more than 4,000 reference strains. Groundwater samples were dominated by gram-negative bacteria of the genera Pseudomonas, Comamonas, Alcaligenes, and Acinetobacter, which were also found in soil samples. In addition, more gram-positive bacteria belonging to the genera Arthrobacter, Nocardia, and Bacillus could be isolated from soil samples.