CHARACTERIZATION OF A METALLOPROTEASE FROM PSYCHROPHILIC XANTHOMONAS-MALTOPHILIA

被引：50

作者：

MARGESIN, R

SCHINNER, F

机构：

来源：

FEMS MICROBIOLOGY LETTERS | 1991年 / 79卷 / 2-3期

关键词：

XANTHOMONAS-MALTOPHILIA; METALLOPROTEASE; PSYCHROPHILIC; ALPINE ENVIRONMENT;

D O I：

10.1016/0378-1097(91)90095-R

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An extracellular protease from psychrophilic Xanthomonas maltophilia isolated from alpine environment was purified and characterized. In spite of a comparable growth at 10-degrees-C and 20-degrees-C, protease excretion occurred only at 10-degrees-C. The enzyme was a 21-kDa protein with an isoelectric point higher than 9.5. The optimum pH and temperature for azocaseinolytic activity were 8.0 and 50-degrees-C respectively. The enzyme was stable up to 40-degrees-C but became inactive after 10 min at 60-degrees-C. The energy of activation was comparable to that of enzymes from mesophilic sources. Sensitivity to EDTA indicates that X. maltophilia protease is a metalloprotease.

引用

页码：257 / 262

页数：6

共 14 条

[1] SOME PROPERTIES OF EXTRACELLULAR PROTEASE PRODUCED BY PSYCHROTROPHIC BACTERIUM PSEUDOMONAS-FLUORESCENS STRAIN AR-11 [J].

ALICHANIDIS, E ;

ANDREWS, AT .

BIOCHIMICA ET BIOPHYSICA ACTA, 1977, 485 (02) :424-433

[2] PURIFICATION AND PROPERTIES OF A SERINE PROTEASE FROM PSEUDOMONAS-MALTOPHILIA [J].

BOETHLING, RS .

JOURNAL OF BACTERIOLOGY, 1975, 121 (03) :933-941

[3]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[4] PURIFICATION AND SOME PROPERTIES OF AN EXTRACELLULAR PROTEASE (CALDOLYSIN) FROM AN EXTREME THERMOPHILE [J].

COWAN, DA ;

DANIEL, RM .

BIOCHIMICA ET BIOPHYSICA ACTA, 1982, 705 (03) :293-305

[5]

FELLER G, 1990, FEMS MICROBIOL LETT, V66, P239, DOI 10.1016/0378-1097(90)90290-7

[6] ISOLATION AND CHARACTERIZATION OF A STREPTOMYCES C5 MUTANT STRAIN HYPERPRODUCTIVE OF EXTRACELLULAR PROTEASE [J].

GIBB, GD ;

DEKLEVA, ML ;

LAMPEL, JS ;

STROHL, WR .

BIOTECHNOLOGY LETTERS, 1987, 9 (09) :605-610

[7]

KATO N, 1972, AGR BIOL CHEM TOKYO, V36, P1117

[8] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[9] PSYCHROPHILIC BACTERIA [J].

MORITA, RY .

BACTERIOLOGICAL REVIEWS, 1975, 39 (02) :144-167

[10] PURIFICATION AND PROPERTIES OF AN EXTRACELLULAR PROTEINASE OF PSYCHROPHILIC ESCHERICHIA-FREUNDII [J].

NAKAJIMA, M ;

MIZUSAWA, K ;

YOSHIDA, F .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1974, 44 (01) :87-96

← 1 2 →