OSTEOCALCIN CLUSTER - IMPLICATIONS FOR FUNCTIONAL-STUDIES

被引：54

作者：

DESBOIS, C ^{[1
]}

KARSENTY, G ^{[1
]}

机构：

[1] UNIV TEXAS, MD ANDERSON CANC CTR, DEPT MOLEC GENET, HOUSTON, TX 77030 USA

来源：

JOURNAL OF CELLULAR BIOCHEMISTRY | 1995年 / 57卷 / 03期

关键词：

BONE EXTRACELLULAR MATRIX; CELL PROLIFERATION; CELL DIFFERENTIATION; OSTEOBLAST; ODONTOBLAST; OSTEOCALCIN; GLA PROTEIN;

D O I：

10.1002/jcb.240570302

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Osteocalcin is a skeletal member of the family of extracellular mineral binding Gla protein. Osteocalcin is synthesized only by the osteoblast and it is secreted into the bone matrix at the time of bone mineralization. The mineral binding properties of osteocalcin as well as its spatial and temporal pattern of expression suggest that it plays a role during bone mineralization, however until now its biological function is unclear. To understand osteocalcin function during skeletogenesis we mutated the two osteocalcin genes by homologous recombination in embryonic stem (ES) cells. Eight targeted clones were identified by Southern analysis using external probes. One of these clones contributed to the germ line of mouse chimera. Interbreeding of heterozygotes is currently in progress. Mutant mice will be useful to understand osteocalcin function in vivo. (C) 1995 Wiley-Liss, Inc.

引用

页码：379 / 383

页数：5

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