PREPARATION AND PROPERTIES OF BRUSH-BORDER MEMBRANE-VESICLES FROM HUMAN SMALL-INTESTINE

被引：91

作者：

SHIRAZIBEECHEY, SP

DAVIES, AG

TEBBUTT, K

DYER, J

ELLIS, A

TAYLOR, CJ

FAIRCLOUGH, P

BEECHEY, RB

机构：

[1] BRONGLAIS GEN HOSP,ABERYSTWYTH,DYFED,WALES

[2] BROADGREEN HOSP,DEPT GASTROENTEROL,LIVERPOOL L14 3LB,MERSEYSIDE,ENGLAND

[3] CHILDRENS HOSP,DEPT PAEDIAT GASTROENTEROL,SHEFFIELD S10 2TH,S YORKSHIRE,ENGLAND

[4] ST BARTHOLOMEWS HOSP,DEPT GASTROENTEROL,LONDON EC1A 7BE,ENGLAND

来源：

GASTROENTEROLOGY | 1990年 / 98卷 / 03期

关键词：

D O I：

10.1016/0016-5085(90)90288-C

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

This study describes a simple and rapid method for the preparation of brush-border membrane vesicles from intestinal biopsies. The specific activities of sucrase, amino peptidase N, and alkaline phosphatase in these vesicles were the same as those in vesicles prepared from intestinal segments. The vesicles from all the regions of the small intestine can transport d-glucose in an Na+-dependent manner. The rates of transport of d-glucose presented here are far higher than previously reported. The method should have a wide applicability to studies of transport mechanisms and the distribution of transport processes within the intestine. © 1990.

引用

页码：676 / 685

页数：10

共 29 条

[1] TAUROCHOLATE TRANSPORT BY HUMAN ILEAL BRUSH-BORDER MEMBRANE-VESICLES [J].