TUMOR METASTASES AND CELL-MEDIATED-IMMUNITY IN A MODEL SYSTEM IN DBA-2 MICE .1. TUMOR INVASIVENESS INVITRO AND METASTASIS FORMATION INVIVO

A syngeneic model system for the study of tumor metastases and cell‐mediated immunity is described. The system consists of two related, chemically induced murine lymphomas, the non‐metastasizing parental line Eb and its metastasizing variant ESb. An unrelated, chemically induced tumor (MDAY) is included for specificity controls. Serological typing revealed that both Eb and ESb were of T lymphoid origin and expressed the H‐2K and H‐2D molecules of the host strain DBA/2. By various electron microscope techniques, morphological differences were observed between the two cell lines. In comparison to Eb cells, ESb tumor cells had a more polymorphic nucleus with many in vagi‐nations of the nuclear envelope and a more prominent expression of microvilli on the cell surface. An in vitro organ culture test for tumor invasiveness, presented here for the first time in a syngeneic murine system, revealed that ESb but not Eb tumor cells had the ability to attach to and invade normal tissue. Accordingly, ESb tumor cells showed higher malignancy in vivo. This was apparent from their higher tumorigenicity and their ability to disseminate and metastasize and to kill recipient mice more quickly. Upon histological examination of the local primary tumors a striking difference was noticed with regard to the degree of infiltration by host‐derived mononuclear cells, mostly histiocytes. The non‐metastasizing tumor Eb was heavily infiltrated while tumor ESb contained only a few of these cells. The differences between the tumor lines ESb and Eb are considered in the light of their possible relevance for metastases in general. The etiology of the two tumors is discussed in particular with respect to their relatedness. Copyright © 1979 Wiley‐Liss, Inc., A Wiley Company