COMPUTER-PROGRAMS FOR THE ASSEMBLY OF DNA SEQUENCES

被引：68

作者：

GINGERAS, TR ^{[1
]}

MILAZZO, JP ^{[1
]}

SCIAKY, D ^{[1
]}

ROBERTS, RJ ^{[1
]}

机构：

[1] SUNY STONY BROOK,CTR COMP,STONY BROOK,NY 11794

来源：

NUCLEIC ACIDS RESEARCH | 1979年 / 7卷 / 02期

关键词：

D O I：

10.1093/nar/7.2.529

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A collection of user-interactive computer programs is described which aid in the assembly of DNA sequences. This is achieved by searching for the positions of overlapping common nucleotide sequences within the blocks of sequence obtained as primary data. Such overlapping segments are then melded into one continuous string of nucleotides. Strategies for determining the accuracy of the sequence being analyzed and reducing the error rate resulting from the manual manipulation of sequence data are discussed. Sequences mapping from 97.3 to 100% of the Ad2 virus genome were used to demonstrate the performance of these programs. © 1979 Information Retrieval Limited.

引用

收藏

页码：529 / 545

页数：17

相关论文

共 12 条

[1] COMPLETE NUCLEOTIDE-SEQUENCE OF SV40 DNA [J].

FIERS, W ;

CONTRERAS, R ;

HAEGEMAN, G ;

ROGIERS, R ;

VANDEVOORDE, A ;

VANHEUVERSWYN, H ;

VANHERREWEGHE, J ;

VOLCKAERT, G ;

YSEBAERT, M .

NATURE, 1978, 273 (5658) :113-120

[2] COMPUTER-ANALYSIS OF NUCLEIC-ACID REGULATORY SEQUENCES [J].

KORN, LJ ;

QUEEN, CL ;

WEGMAN, MN .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (10) :4401-4405

[3] NEW METHOD FOR SEQUENCING DNA [J].

MAXAM, AM ;

GILBERT, W .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (02) :560-564

[4] CLEAVAGE MAPS OF DNA FROM ADENOVIRUS TYPES 2 AND 5 BY RESTRICTION ENDONUCLEASES ECORI AND HPAI [J].

MULDER, C ;

ARRAND, JR ;

DELIUS, H ;

KELLER, W ;

PETTERSSON, U ;

ROBERTS, RJ ;

SHARP, PA .

COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 1974, 39 :397-400

[5]

REDDY VB, 1978, SCIENCE, V200, P494, DOI 10.1126/science.205947

[6]

ROBERTS RJ, 1979, METHODS ENZYMOLOGY, V65

[7] RAPID METHOD FOR DETERMINING SEQUENCES IN DNA BY PRIMED SYNTHESIS WITH DNA-POLYMERASE [J].

SANGER, F ;

COULSON, AR .

JOURNAL OF MOLECULAR BIOLOGY, 1975, 94 (03) :441-+

[8] NUCLEOTIDE-SEQUENCE OF BACTERIOPHAGE PHICHI174 DNA [J].

SANGER, F ;

AIR, GM ;

BARRELL, BG ;

BROWN, NL ;

COULSON, AR ;

FIDDES, JC ;

HUTCHISON, CA ;

SLOCOMBE, PM ;

SMITH, M .

NATURE, 1977, 265 (5596) :687-695

[9] DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS [J].

SANGER, F ;

NICKLEN, S ;

COULSON, AR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) :5463-5467

[10] SIMPLE METHOD FOR DNA RESTRICTION SITE MAPPING [J].

SMITH, HO ;

BIRNSTIEL, ML .

NUCLEIC ACIDS RESEARCH, 1976, 3 (09) :2387-2398