STRUCTURE OF THE HAFNIA-ALVEI STRAIN PCM-1188 O-SPECIFIC POLYSACCHARIDE

被引：11
作者：
GAMIAN, A
KATZENELLENBOGEN, E
ROMANOWSKA, E
FERNANDEZ, JMG
PEDERSEN, C
ULRICH, J
DEFAYE, J
机构：
[1] UNIV SEVILLA, FAC QUIM, DEPT QUIM ORGAN, E-41071 SEVILLE, SPAIN
[2] TECH UNIV DENMARK, INST ORGAN CHEM, DK-2800 LYNGBY, DENMARK
[3] CNRS, F-38027 GRENOBLE, FRANCE
[4] CEA, INST BIOL STRUCT, LSMP, F-38027 GRENOBLE, FRANCE
[5] CNRS, F-38054 GRENOBLE, FRANCE
[6] CEA, DEPT RECH FONDAMENTALE MAT CONDENSEE, SESAM, CTR ETUD GRENOBLE, F-38054 GRENOBLE, FRANCE
来源：
CARBOHYDRATE RESEARCH | 1995年 / 277卷 / 02期
关键词：
LIPOPOLYSACCHARIDE; HAFNIA ALVEI; ENDOTOXIN; LPS; O-ANTIGEN;
D O I：
10.1016/0008-6215(95)00200-D
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The lipopolysaccharide was extracted from cells of Hafnia alvei PCM 1188 strain and, after mild acid hydrolysis, the O-specific polysaccharide isolated and characterized. On the basis of sugar and methylation analysis, FAB mass spectrometry and NMR spectroscopy of the polysaccharide and oligosaccharides obtained after Smith degradation, or solvolysis with anhydrous hydrogen fluoride, the repeating unit of the O-specific polysaccharide was shown to be the pentasaccharide: [GRAPHICS] --> 4)-beta-D-Gal p-(1 --> 3)-beta-D-Glc pNAc-(1 --> 4)-beta-D-Glc pUA-(1 --> 2)-alpha-D-Man p-(1 -->
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收藏
页码：245 / 255
页数：11
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