ALCOHOL-DEHYDROGENASE (ADH2) FROM A MUTANT STRAIN OF CANDIDA-GUILLIERMONDII A80-03 - PURIFICATION AND CHARACTERIZATION

被引：0

作者：

INDRATI, R ^{[1
]}

OHTA, Y ^{[1
]}

机构：

[1] HIROSHIMA UNIV,FAC APPL BIOL SCI,MICROBIAL BIOCHEM LAB,4-4 KAGAMIYAMA 1 CHOME,HIGASHI,HIROSHIMA 724,JAPAN

来源：

FEMS MICROBIOLOGY LETTERS | 1993年 / 110卷 / 02期

关键词：

CANDIDA-GUILLIERMONDII; DIMER; ALCOHOL DEHYDROGENASE; ADH2;

D O I：

暂无

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Alcohol dehydrogenase ADH2 was purified twice from Candida guilliermondii strain A80-03, by ion exchange column chromatography on DEAE-Toyopearl 650M. The enzyme was a dimer of M(r) 98500. ADH2 had a broad substrate specificity, oxidizing secondary alcohols as well as primary alcohols. The enzyme was sensitive to several inhibitors, such as metal chelators and thiol reagents. Kinetic studies suggested that ADH2 oxidized ethanol by an iso ordered sequential mechanism.

引用

页码：179 / 184

页数：6

共 24 条

[1] Bergmeyer H., 1974, METHOD ENZYMAT AN, V1, P428
[2] BICSAK TA, 1982, ARCH BIOCHEM BIOPHYS, V126, P605
[3] KINETICS OF ENZYME-CATALYZED REACTIONS WITH 2 OR MORE SUBSTRATES OR PRODUCTS .1. NOMENCLATURE AND RATE EQUATIONS
CLELAND, WW
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1963, 67 (01) : 104 - &
[4] SIMIAN LIVER ALCOHOL-DEHYDROGENASE - ISOLATION AND CHARACTERIZATION OF ISOENZYMES FROM SAIMIRI-SCIUREUS
DAFELDECKER, WP
PARES, X
VALLEE, BL
BOSRON, WF
LI, TK
[J]. BIOCHEMISTRY, 1981, 20 (04) : 856 - 861
[5] ROLE OF ESSENTIAL THIOL GROUPS OF YEAST ALCOHOL-DEHYDROGENASE
DICKINSON, FM
[J]. BIOCHEMICAL JOURNAL, 1972, 126 (01) : 133 - +
[6] DICKSINSON M, 1977, BIOCHEM J, V167, P237
[7] DIKINSON FM, 1970, BIOCHEM J, V120, P821
[8] GUILBAULT GG, 1976, HDB ENZYMATIC METHOD, P64
[9] SUBUNIT COMPOSITION OF ALCOHOL-DEHYDROGENASE FROM THEA-SINENSIS SEEDS AND ITS SUBSTRATE-SPECIFICITY FOR MONOTERPENES
HATANAKA, A
SEKIYA, J
KAJIWARA, T
[J]. PHYTOCHEMISTRY, 1976, 15 (04) : 487 - 488
[10] COENZYME-DEPENDENT ALCOHOL DEHYDROGENASES IN CANDIDA-GUILLIERMONDII Y4
INDRATI, R
NAITO, J
OHTA, Y
[J]. CANADIAN JOURNAL OF MICROBIOLOGY, 1991, 37 (11) : 803 - 807

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