LYSINE 2,3-AMINOMUTASE - ROLE OF S-ADENOSYL-L-METHIONINE IN THE MECHANISM - DEMONSTRATION OF TRITIUM TRANSFER FROM (2RS,3RS)-[3-H-3]LYSINE TO S-ADENOSYL-L-METHIONINE

被引：18

作者：

KILGORE, JL ^{[1
]}

ABERHART, DJ ^{[1
]}

机构：

[1] WORCESTER FDN EXPTL BIOL INC,SHREWSBURY,MA 01545

来源：

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1 | 1991年 / 01期

关键词：

D O I：

10.1039/p19910000079

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

(2RS,3RS)-[3-H-3]Lysine has been synthesized, and incubated with lysine 2,3-aminomutase in the presence of S-adenosyl-L-methionine (SAM). After incubation, the SAM was converted, by heating, into methylthioadenosine (MTA). The latter contained ca. 1-6% of the incubated tritium, consistent with an exchange of SAM hydrogens (probably at C-5') with the 3-pro-R hydrogen of lysine. The results support a mechanism for lysine 2,3-aminomutase in which SAM acts as hydrogen carrier.

引用

页码：79 / 84

页数：6

共 26 条

[1] SEPARATION BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF ALPHA-AMINO AND BETA-AMINO ACIDS - APPLICATION TO ASSAYS OF LYSINE 2,3-AMINOMUTASE AND LEUCINE 2,3-AMINOMUTASE [J].