ACUTE STIMULATION BY LUTROPIN OF MITOCHONDRIAL PROTEIN-SYNTHESIS IN SMALL LUTEAL CELLS

被引：7

作者：

MITTRE, H ^{[1
]}

AUNAI, P ^{[1
]}

BENHAIM, A ^{[1
]}

LEYMARIE, P ^{[1
]}

机构：

[1] CHU COTE NACRE,BIOCHIM LAB,CNRS,UA 609,F-14032 CAEN,FRANCE

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1990年 / 187卷 / 03期

关键词：

D O I：

10.1111/j.1432-1033.1990.tb15359.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A two‐dimensional electrophoretic technique was used to study the effect of acute stimulation of bovine luteal cells with lutropin on protein synthesis. Cells were incubated for 30 min with [35S]methionine in the presence of stimulating levels of luteinizing hormone (lutropin), after which the proteins were analyzed by autoradiography. Lutropin or N6,2′‐O‐dibutyryl‐adenosine 3′,5′‐phosphate (Bt2cAMP) induced the labelling of three proteins, referred to as proteins A, B and C. Protein A, had a molecular mass of 28 kDa and an isoelectric point (pI) of 6.7. Proteins B and C had a molecular mass of 27 kDa and pI of 6.2 and 6.4 respectively. After subcellular fractionation, the three proteins were found to be markedly concentrated in the only fraction enriched in an established mitochondrial marker. Moreover, protein A was one of the major mitochondrial newly synthesized proteins. Its appearance was observed after a 5‐min incubation and was prevented by 100 μM cycloheximide. The acute accumulation of proteins A, B and C in mitochondria, the site of the rate‐limiting step of steroidogenesis, suggest that they could be involved in the mechanism of stimulation by lutropin of progesterone synthesis. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：721 / 726

页数：6