APPARENT NONCOMPETITIVE ANTAGONISM OF MUSCARINIC RECEPTOR MEDIATED CA2+ MOBILIZATION BY SOME MUSCARINIC ANTAGONISTS

被引：11

作者：

KUKKONEN, J

AKERMAN, KEO

机构：

[1] Department of Biochemistry and Pharmacy, Åbo Akademi University, SF-20521 Turku, Biocity

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 189卷 / 02期

关键词：

D O I：

10.1016/0006-291X(92)92291-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ca2+ mobilizations in SH-SY5Y and IMR-32 human neuroblastoma cell lines were measured using the fluorescent Ca2+ indicator fura-2. A variety of antagonists (atropine, pirenzepine, 4-DAMP and N-methyl-scopolamine) inhibited carbamyl choline-induced transient Ca2+ mobilization both in a competitive and a noncompetitive manner. The apparent noncompetitive inhibition constants were lower in IMR-32 than in SH-SY5Y cells even when the competitive inhibition constants were similar. This may relate to the previously reported differential expression of muscarinic receptor subtypes in these cell lines. © 1992.

引用

页码：919 / 924

页数：6

共 24 条

[1] 12-O-TETRADECANOYLPHORBOL 13-ACETATE AND FORSKOLIN MODIFY MUSCARINIC RECEPTOR-LINKED CA-2+ MOBILIZATION IN SH-SY5Y NEUROBLASTOMA-CELLS THROUGH DIFFERENT MECHANISMS [J].