OCCURRENCE OF LIPIDIC PARTICLES IN LIPID BILAYERS AS SEEN BY P-31-NMR AND FREEZE-FRACTURE ELECTRON-MICROSCOPY

被引：192

作者：

DEKRUIJFF, B ^{[1
]}

VERKLEY, AJ ^{[1
]}

VANECHTELD, CJA ^{[1
]}

GERRITSEN, WJ ^{[1
]}

MOMBERS, C ^{[1
]}

NOORDAM, PC ^{[1
]}

DEGIER, J ^{[1
]}

机构：

[1] STATE UNIV UTRECHT, DEPT BIOCHEM, UTRECHT, NETHERLANDS

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 555卷 / 02期

关键词：

(Inverted micelle); !sup]31[!/sup]P-NMR; Freeze fracture; Lipid particles;

D O I：

10.1016/0005-2736(79)90160-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new type of lipid organization is observed in mixtures of phosphatidylcholine with cardiolipin (in the presence of Ca2+), monoglucosyldiglyceride and phosphatidylethanolamine (in the presence of cholesterol). This phase is characterised by an isotropic 31P NMR signal and is visualised by freeze-fracturing as particles and pits on the fracture faces of the lipid bilayer. As the most favourable model for this phase we propose the inverted micelle sandwiched in between the two monolayers of the lipid bilayer. © 1979.

引用

页码：200 / 209

页数：10

共 26 条

[1] EFFECTS OF FUSOGENIC AGENT ON MEMBRANE STRUCTURE OF ERYTHROCYTE-GHOSTS AND MECHANISM OF MEMBRANE-FUSION [J].

CULLIS, PR ;

HOPE, MJ .

NATURE, 1978, 271 (5646) :672-674

[2] P-31 NMR-STUDIES OF UNSONICATED AQUEOUS DISPERSIONS OF NEUTRAL AND ACIDIC PHOSPHOLIPIDS - EFFECTS OF PHASE-TRANSITIONS, P2H AND DIVALENT-CATIONS ON MOTION IN PHOSPHATE REGION OF POLAR HEADGROUP [J].

CULLIS, PR ;

DEKRUYFF, B .

BIOCHIMICA ET BIOPHYSICA ACTA, 1976, 436 (03) :523-540

[3] EFFECTS OF CHOLESTEROL ON PROPERTIES OF EQUIMOLAR MIXTURES OF SYNTHETIC PHOSPHATIDYLETHANOLAMINE AND PHOSPHATIDYLCHOLINE - P-31 NMR AND DIFFERENTIAL SCANNING CALORIMETRY STUDY [J].

CULLIS, PR ;

VANDIJCK, PWM ;

DEKRUIJFF, B ;

DEGIER, J .

BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 513 (01) :21-30

[4] POLYMORPHIC PHASE BEHAVIOR OF LIPID MIXTURES AS DETECTED BY P-31 NMR - EVIDENCE THAT CHOLESTEROL MAY DESTABILIZE BILAYER STRUCTURE IN MEMBRANE SYSTEMS CONTAINING PHOSPHATIDYLETHANOLAMINE [J].

CULLIS, PR ;

DEKRUIJFF, B .

BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 507 (02) :207-218

[5] POLYMORPHIC PHASE BEHAVIOR OF PHOSPHATIDYLETHANOLAMINES OF NATURAL AND SYNTHETIC ORIGIN - P-31 NMR-STUDY [J].

CULLIS, PR ;

DEKRUIJFF, B .

BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 513 (01) :31-42

[6] POLYMORPHIC PHASE BEHAVIOR OF CARDIOLIPIN AS DETECTED BY P-31 NMR AND FREEZE-FRACTURE TECHNIQUES - EFFECTS OF CALCIUM, DIBUCAINE AND CHLORPROMAZINE [J].

CULLIS, PR ;

VERKLEIJ, AJ ;

VERVERGAERT, PHJT .

BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 513 (01) :11-20

[7] LATERAL DIFFUSION RATES OF PHOSPHATIDYLCHOLINE IN VESICLE MEMBRANES - EFFECTS OF CHOLESTEROL AND HYDROCARBON PHASE-TRANSITIONS [J].

CULLIS, PR .

FEBS LETTERS, 1976, 70 (01) :223-228

[8] LARGE VOLUME LIPOSOMES BY AN ETHER VAPORIZATION METHOD [J].

DEAMER, D ;

BANGHAM, AD .

BIOCHIMICA ET BIOPHYSICA ACTA, 1976, 443 (03) :629-634

[9] LAMELLAR AND HEXAGONAL LIPID PHASES VISUALIZED BY FREEZE-ETCHING [J].

DEAMER, DW ;

LEONARD, R ;

TARDIEU, A ;

BRANTON, D .

BIOCHIMICA ET BIOPHYSICA ACTA, 1970, 219 (01) :47-&

[10] OUTSIDE-INSIDE DISTRIBUTIONS AND SIZES OF MIXED PHOSPHATIDYLCHOLINE-CHOLESTEROL VESICLES [J].

DEKRUIJFF, B ;

CULLIS, PR ;

RADDA, GK .

BIOCHIMICA ET BIOPHYSICA ACTA, 1976, 436 (04) :729-740

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