MASS MEASUREMENT OF INOSITOL 1,4,5-TRISPHOSPHATE AND SN-1,2-DIACYLGLYCEROL IN BOMBESIN-STIMULATED SWISS 3T3 MOUSE FIBROBLASTS

被引：71

作者：

COOK, SJ ^{[1
]}

PALMER, S ^{[1
]}

PLEVIN, R ^{[1
]}

WAKELAM, MJO ^{[1
]}

机构：

[1] UNIV GLASGOW,DEPT BIOCHEM,MOLEC PHARMACOL GRP,GLASGOW G12 8QQ,SCOTLAND

来源：

BIOCHEMICAL JOURNAL | 1990年 / 265卷 / 02期

关键词：

D O I：

10.1042/bj2650617

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two specific and selective assays were used to measure changes in the mass of Ins(1,4,5)P3 and sn-1,2-diacylglycerol in bombesin-stimulated Swiss 3T3 cells. The results demonstrate that the increase in Ins(1,4,5)P3 was extremely rapid, but transient, returning to basal levels by 30 s. In contrast, the increase in sn-1,2-diacylglycerol was biphasic: the first phase mirrored the transient Ins(1,4,5)P3 response, whereas the second phase was sustained and occurred in the absence of elevated Ins(1,4,5)P3. The possible source of the second phase of diacylglycerol is discussed.

引用

页码：617 / 620

页数：4

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