ION-EXCHANGE COLUMN CHROMATOGRAPHY OF DESMOSINE AND ISODESMOSINE

A modification of a standard amino acid analysis procedure is used for the resolution and quantification of the polyfunctional amino acids desmosine and isodesmosine. In addition to the long and short columns normally used during an amino acid analysis, a third column (60 cm resin height) packed with Beckman Custom Research Resin type PA-28 is used to resolve desmosine and isodesmosine. These two amino acids are eluted with a 0.2 N sodium citrate buffer, pH 5.28 ± 0.02. © 1969.