THE COMET ASSAY - A COMPREHENSIVE REVIEW

被引：1361
|
作者：
FAIRBAIRN, DW
OLIVE, PL
ONEILL, KL
机构：
[1] BRIGHAM YOUNG UNIV, DEPT MICROBIOL, PROVO, UT 84602 USA
[2] BRITISH COLUMBIA CANC RES CTR, VANCOUVER, BC V5Z 1L3, CANADA
来源：
MUTATION RESEARCH-REVIEWS IN GENETIC TOXICOLOGY | 1995年 / 339卷 / 01期
关键词：
COMET ASSAY; SINGLE CELL GEL; MICROGEL ELECTROPHORESIS; DNA SINGLE STRAND BREAKS; DOUBLE STRAND BREAKS;
D O I：
10.1016/0165-1110(94)00013-3
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The comet assay is a sensitive and rapid method for DNA strand break detection in individual cells. Its use has increased significantly in the past few years. This paper is a review of the studies published to date that have made use of the comet assay. The principles of strand break detection using both the alkaline and neutral versions of the technique are discussed, and a basic methodology with currently used variations is presented. Applications in different fields are reviewed and possible future directions of the technique are briefly explored.
引用
收藏
页码：37 / 59
页数：23
相关论文
共 50 条
  • [41] Comet Assay in Neonatal Sepsis
    Carvalho, Natalia C.
    de Souza, Rozilda L.
    Dal-Pizzol, Felipe
    Andrade, Vanessa M.
    INDIAN JOURNAL OF PEDIATRICS, 2010, 77 (08): : 875 - 877
  • [42] Impact of the comet assay in radiobiology
    Olive, Peggy L.
    MUTATION RESEARCH-REVIEWS IN MUTATION RESEARCH, 2009, 681 (01) : 13 - 23
  • [43] COMET ASSAY - TECHNICAL ASPECTS
    ALAPETITE, C
    LEVY, E
    MOUSTACCHI, E
    PATHOLOGIE BIOLOGIE, 1994, 42 (10): : 930 - 931
  • [44] Novel formats for the comet assay
    Shaposhnikov, Sergey
    Brunborg, Gunnar
    Azqueta, Amaya
    Gaivao, Isabel
    Smart, Andrew
    Collins, Andrew R.
    TOXICOLOGY LETTERS, 2013, 221 : S189 - S189
  • [45] Detection of crosslinks with the comet assay
    Speit, G
    Merk, O
    NEOPLASMA, 1999, 46 : 76 - 78
  • [46] Application of the yeast comet assay in testing some food additives for genotoxicity by comet assay in yeast
    Rashad, Shimaa E.
    Abdel-Tawab, F. M.
    Fahmy, Eman M.
    Attalla, Ashraf G.
    Ahmed, Ekram S.
    Haggran, A. A.
    EGYPTIAN JOURNAL OF CHEMISTRY, 2021, 64 (12): : 7549 - 7567
  • [47] Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results
    Peter Møller
    Amaya Azqueta
    Elisa Boutet-Robinet
    Gudrun Koppen
    Stefano Bonassi
    Mirta Milić
    Goran Gajski
    Solange Costa
    João Paulo Teixeira
    Cristiana Costa Pereira
    Maria Dusinska
    Roger Godschalk
    Gunnar Brunborg
    Kristine B. Gutzkow
    Lisa Giovannelli
    Marcus S. Cooke
    Elke Richling
    Blanca Laffon
    Vanessa Valdiglesias
    Nursen Basaran
    Cristian Del Bo’
    Bojana Zegura
    Matjaz Novak
    Helga Stopper
    Pavel Vodicka
    Sona Vodenkova
    Vanessa Moraes de Andrade
    Monika Sramkova
    Alena Gabelova
    Andrew Collins
    Sabine A. S. Langie
    Nature Protocols, 2020, 15 : 3817 - 3826
  • [48] Increasing the resolution of the comet assay using fluorescent in situ hybridization-a review
    Shaposhnikov, Sergey
    Frengen, Eirik
    Collins, Andrew R.
    MUTAGENESIS, 2009, 24 (05) : 383 - 389
  • [49] Evaluation of Genotoxic Effects of Xenobiotics in Fishes Using Comet Assay-A Review
    Machado Da Rocha, Carlos Alberto
    Lima De Lima, Patricia Danielle
    Dos Santos, Raquel Alves
    Rodriguez Burbano, Rommel Mario
    REVIEWS IN FISHERIES SCIENCE, 2009, 17 (02): : 170 - 173
  • [50] A scoping review of recent advances in the application of comet assay to Allium cepa roots
    Alias, Carlotta
    Zerbini, Ilaria
    Feretti, Donatella
    ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 2023, 64 (05) : 264 - 281
12345