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DETECTION OF CELL-CYCLE STAGE BY FLUORESCENCE INSITU HYBRIDIZATION - ITS APPLICATION IN HUMAN INTERPHASE CYTOGENETICS
被引:24
作者:
MUKHERJEE, AB
[1
]
MURTY, VVVS
[1
]
CHAGANTI, RSK
[1
]
机构:
[1] SLOAN KETTERING MEM CANC CTR,CANC GENET LAB,NEW YORK,NY 10021
来源:
CYTOGENETICS AND CELL GENETICS
|
1992年
/
61卷
/
02期
关键词:
D O I:
10.1159/000133378
中图分类号:
Q2 [细胞生物学];
学科分类号:
071009 ;
090102 ;
摘要:
Distinct cell-cycle-dependent changes in the conformation of centromeric chromatin in a specific human chromosome containing alpha-satellite DNA have been demonstrated by fluorescence in situ hybridization (FISH). This method, based upon specific FISH signal morphology, allows simultaneous analysis of chromosomal aneuploidy and detection of specific cell-cycle stage(s) of human tumor and/or normal cell populations in a single preparation of interphase cells. This interphase cytogenetic procedure might prove useful for both basic and clinical research involving human cells.
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页码:91 / 94
页数:4
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