DETECTION OF CELL-CYCLE STAGE BY FLUORESCENCE INSITU HYBRIDIZATION - ITS APPLICATION IN HUMAN INTERPHASE CYTOGENETICS

被引：24

作者：

MUKHERJEE, AB ^{[1
]}

MURTY, VVVS ^{[1
]}

CHAGANTI, RSK ^{[1
]}

机构：

[1] SLOAN KETTERING MEM CANC CTR,CANC GENET LAB,NEW YORK,NY 10021

来源：

CYTOGENETICS AND CELL GENETICS | 1992年 / 61卷 / 02期

关键词：

D O I：

10.1159/000133378

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Distinct cell-cycle-dependent changes in the conformation of centromeric chromatin in a specific human chromosome containing alpha-satellite DNA have been demonstrated by fluorescence in situ hybridization (FISH). This method, based upon specific FISH signal morphology, allows simultaneous analysis of chromosomal aneuploidy and detection of specific cell-cycle stage(s) of human tumor and/or normal cell populations in a single preparation of interphase cells. This interphase cytogenetic procedure might prove useful for both basic and clinical research involving human cells.

引用

页码：91 / 94

页数：4

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