HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF OLIGOSACCHARIDE ALDITOLS AND GLYCOPEPTIDES ON A GRAPHITIZED CARBON COLUMN

被引：74

作者：

DAVIES, M ^{[1
]}

SMITH, KD ^{[1
]}

HARBIN, AM ^{[1
]}

HOUNSELL, EF ^{[1
]}

机构：

[1] CLIN RES CTR, GLYCOCONJUGATES SECT, WATFORD RD, HARROW HA1 3UJ, MIDDX, ENGLAND

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 609卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(92)80155-N

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The chromatographic behaviour of oligosaccharide alditols and glycopeptides containing neutral and acetamido sugars and sialic acid has been investigated on a HyperCarb porous graphitised carbon column. The alditols were substantially retained and could be eluted in 0-25% acetonitrile-0.05% trifluoroacetic acid in 0.05% aqueous trifluoroacetic acid between 3-30 min for mono- to hexasaccharides. Elution patterns were based on both size, charge and linkage such that isomeric compounds could be separated from each other.

引用

页码：125 / 131

页数：7

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